Daniel Knowland was the lab manager for the Agalliu lab from February 2012 until August 2013. Daniel is currently a graduate student in Neuroscience at UCSD. He received his B.S. in Neuroscience from UCLA in 2011. His scientific interests include all subjects related to the brain. In addition to day-to-day managerial duties, he was also involved with two projects in the Agalliu lab. First, he was seeking to elucidate the molecular mechanisms underlying blood-brain barrier (BBB) breakdown after stroke in a rodent model utilizing novel transgenic mice and two-photon microscopy. Secondly, he was looking to understand the process by which children develop neuropsychiatric disorders after a streptococcal infection. This affliction is more commonly referred to as pediatric neuropsychiatric disorders associated with streptococcus (PANDAS). Specifically, he is investigating the role of the compromised BBB and its relation to T-cell entry into the brain in the disease state.